The CIA and Federal Bureau of Investigation, whose records make up the bulk of the batch, won't say whether they've appealed to the Republican president to keep them under wraps.
Pending the President's possible interjection, the National Archives will release the files on October 26.
"Subject to the receipt of further information, I will be allowing, as President, the long blocked and classified JFK FILES to be opened", Trump said in a tweet.
Trump's announcement comes after a number of government agencies requested he hold off on making the documents public, lest they expose relatively recent intelligence and law enforcement operations.
Most of the assassination documents (88%) have already been released, according to a JFK-related Web site that has been counting down the days, with the remainder now being released next week comprising 3,810 documents, including formerly redacted and withheld documents.
Larry Sabato, director of the University of Virginia Centre for Politics and author of a book about Kennedy tweeted the president: "Thank you".More news: Apple Inc. (AAPL) Lifted to "Overweight" at KeyCorp
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Naturally, scholars and armchair detectives are buzzing at the thought of all these documents being released.
Mr Trump has at times dabbled in other conspiracy theories: He once seemed to consider the possibility that Justice Antonin Scalia had been murdered and for years, he was the most vocal purveyor of the falsehood that former president Barack Obama was born outside the United States. The Warren Commission reported after their 1964 study, that they believed that Lee Harvey Oswald acted alone in the shooting, although some official later studies concluded that the shooting was the result of a conspiracy.
The Washington Post reported that Trump had been urged by government agencies, including the CIA to keep the documents secret.
'Why? Because I believe they show that Oswald was trained, nurtured and put in place by the Central Intelligence Agency.
In July, the National Archives published more than 440 never-before-seen assassination documents and thousands of others that had been released previously with redactions.